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Post by nathalia on Nov 16, 2015 22:08:10 GMT -6
Luteovirus is a genus in the family Luteoviridae, which comprises positive single stranded RNA viruses. The type species of this genus is represented by Barley yellow dwarf virus (BYDV). Members of Luteovirus genus have spherical icosahedral particles and monopartite linear genome with 6 ORFs. They are transmitted by aphids in a circulative manner, and are phloem limited.
Recombination seems to be the driving force of evolution of Luteoviruses. Luteoviruses have frequent intra and interspecific recombinantion. It was estimated between 10-3 to 10-4 nucleotide substitution per site per year. A hypothesis about Luteovirus evolution is that they evolved by recombination in hot spots of RNA recombination that are now known to be associated with sites of transcription of subgenomic RNA. Clusters of recombination sites were observed in the 3’ end of RdRp and CP genes of several luteovirus species. The recombination events in Luteovirus are considered as a form of modular evolution resulted from gene modular exchange with members of the Tombusvirus and Luteovirus ancestors.
I read something interesting. One phylogenetic study revealed that Luteoviridae family possibly arose within the 4,000 years. However, all the individual luteovirus species identified so far appeared only in the last 500 years. This observation is common among plant RNA viruses including luteovirus. One reason behind this ‘recent’ emerging of species could be a result of intensification of agricultural practices that direct drives the arms race.
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Post by ravendra on Nov 18, 2015 4:37:22 GMT -6
Nathalia, thanks for posting. What is the host range of Luteoviruses? Is there any infectious cDNA clone available?
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Post by jontrip247 on Nov 18, 2015 8:59:26 GMT -6
Nathalia, Your virus, use recombination as a mechanism to overcome selective pressures. Do you think that recombination is a more recent evolutionary property or has this virus always been able to do this? My thoughts were that the ability to insert other viral/host plant RNAs might make this system very ancient. This in part is due to the lesser specificity. As a virus, there are many selective pressures (biological, environmental). Early on, you would need a mechanism that could grab anything to improve your progeny survival. As centuries progressed and the environments stabilized you might not need the more advanced mechanisms of DNA/RNA exchange and eventually loose the ability of recombination, reassortment. Although - if I remember correctly > recombination requires recombination elements on each end which are observed in eukaryotes. So, was this developed by the virus and then incorporated by the host or vice versa? Ugh. I have a headache now.  |
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Post by omararias on Nov 18, 2015 16:54:05 GMT -6
Luteovirus is a genus in the family Luteoviridae, which comprises positive single stranded RNA viruses. The type species of this genus is represented by Barley yellow dwarf virus (BYDV). Members of Luteovirus genus have spherical icosahedral particles and monopartite linear genome with 6 ORFs. They are transmitted by aphids in a circulative manner, and are phloem limited. Recombination seems to be the driving force of evolution of Luteoviruses. Luteoviruses have frequent intra and interspecific recombinantion. It was estimated between 10-3 to 10-4 nucleotide substitution per site per year. A hypothesis about Luteovirus evolution is that they evolved by recombination in hot spots of RNA recombination that are now known to be associated with sites of transcription of subgenomic RNA. Clusters of recombination sites were observed in the 3’ end of RdRp and CP genes of several luteovirus species. The recombination events in Luteovirus are considered as a form of modular evolution resulted from gene modular exchange with members of the Tombusvirus and Luteovirus ancestors. I read something interesting. One phylogenetic study revealed that Luteoviridae family possibly arose within the 4,000 years. However, all the individual luteovirus species identified so far appeared only in the last 500 years. This observation is common among plant RNA viruses including luteovirus. One reason behind this ‘recent’ emerging of species could be a result of intensification of agricultural practices that direct drives the arms race. Hi Nathalia, please could you help me with some questions. First of all, do you think that the aphid vector might have had something to do with respect to the evolution manner of this virus family? Secondly, from what you are relating, which specifically will be the strategy that you would apply for coping the disease? Thanks. |
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Post by dulanjani on Nov 18, 2015 19:55:49 GMT -6
Luteovirus is a genus in the family Luteoviridae, which comprises positive single stranded RNA viruses. The type species of this genus is represented by Barley yellow dwarf virus (BYDV). Members of Luteovirus genus have spherical icosahedral particles and monopartite linear genome with 6 ORFs. They are transmitted by aphids in a circulative manner, and are phloem limited. Recombination seems to be the driving force of evolution of Luteoviruses. Luteoviruses have frequent intra and interspecific recombinantion. It was estimated between 10-3 to 10-4 nucleotide substitution per site per year. A hypothesis about Luteovirus evolution is that they evolved by recombination in hot spots of RNA recombination that are now known to be associated with sites of transcription of subgenomic RNA. Clusters of recombination sites were observed in the 3’ end of RdRp and CP genes of several luteovirus species. The recombination events in Luteovirus are considered as a form of modular evolution resulted from gene modular exchange with members of the Tombusvirus and Luteovirus ancestors. I read something interesting. One phylogenetic study revealed that Luteoviridae family possibly arose within the 4,000 years. However, all the individual luteovirus species identified so far appeared only in the last 500 years. This observation is common among plant RNA viruses including luteovirus. One reason behind this ‘recent’ emerging of species could be a result of intensification of agricultural practices that direct drives the arms race. Dear Nataliya Since this virus is phloem limited how can this inter specific recombination occur? (between species?) Dose it require mix infection of other phloem specific plant viruses in the host or dose it occur in the vector? |
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