Post by nathalia on Oct 14, 2015 21:36:41 GMT -6
Tospovirus is a genus in the family Bunyaviridae represented by Tomato Spotted Wilt Virus - TSWV.
Negative single stranded RNA.
Tripartite genome (L, M and S segments) enconde a total of six proteins.
M and S segments are ambisense (ORFs in both directions of the strand/transcription occurs in both directions)
-RNA is transcribed to +RNA upon entry infection of a cell
Transcription/replication is connected with translation: evidence that total purified mRNA produced proteins in vitro, BUT RNA synthesis was independent of translation.
Translation strategies:
a) 5' end of mRNAs is primed by cap snatching, required for mRNA transcriptions and translation
b) M and S segments contain a conserved A/U rich regions between ORFs (intergenic regions) that can form a harpin-like structure (or stem-loop): 5' and 3' ends in contact with each other is considered to be transcription-termination site and to stimulate mRNA translation.
c) S segment has two ORFs that produces N and NSs proteins (nucleocapsid and non-structural, respectively). These two proteins are though to interact with the harpin.
Negative single stranded RNA.
Tripartite genome (L, M and S segments) enconde a total of six proteins.
M and S segments are ambisense (ORFs in both directions of the strand/transcription occurs in both directions)
-RNA is transcribed to +RNA upon entry infection of a cell
Transcription/replication is connected with translation: evidence that total purified mRNA produced proteins in vitro, BUT RNA synthesis was independent of translation.
Translation strategies:
a) 5' end of mRNAs is primed by cap snatching, required for mRNA transcriptions and translation
b) M and S segments contain a conserved A/U rich regions between ORFs (intergenic regions) that can form a harpin-like structure (or stem-loop): 5' and 3' ends in contact with each other is considered to be transcription-termination site and to stimulate mRNA translation.
c) S segment has two ORFs that produces N and NSs proteins (nucleocapsid and non-structural, respectively). These two proteins are though to interact with the harpin.
